Lolu chungechunge lulandisa ngezigigaba zezimfudu ezine kanye nobaba wazo wokutholwa, igundane Splinter, abahlala ezindleleni zendle eNew York. Ukuchayeka ku-mutagen kuguqule zonke ezinhlanu zaba izidalwa ezinjengomuntu. Iqeqeshwe yi-Splinter kubuciko be-Ninjutsu, lezi zimfudu ezine zilwa nezinsongo ezihlukahlukene zedolobha, kungaba ezinye i-ninjas, i-mutants, abafokazi, izigebengu noma ngezinye izikhathi ngisho nezidalwa ezingaphezu kwemvelo, zonke zizama ukufihla ubukhona bazo emphakathini.